Journal: Cell genomics

Article Title: Genome-scale CRISPR screening in a single mouse liver

doi: 10.1016/j.xgen.2022.100217

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: HS-CRM8-TTRmin-dsRed: GAATTCGCTAGCACCGGCGCGCCGGGGGAGGCTGCTGGTGAATATTAACCAAGGTCACCCCAGTTATCGGAGGAGCAAACAGGGGCTAAGTCCACACGCGTGGTACCGTCTGTCTGCACATTTCGTAGAGCGAGTGTTCCGATACTCTAATCTCCCTAGGCAAGGTTCATATTTGTGTAGGTTACTTATTCTCCTTTTGTTGACTAAGTCAATAATCAGAATCAGCAGGTTTGGAGTCAGCTTGGCAGGGATCAGCAGCCTGGGTTGGAAGGAGGGGGTATAAAAGCCCCTTCACCAGGAGAAGCCGTCACACAGATCCACAAGCTCCTGACCGGTTCTAGAGCGCTGCCACCATGGTGCGCTCCTCCAAGAACGTCATCAAGGAGTTCATGCGCTTCAAGGTGCGCATGGAGGGCACCGTGAACGGCCACGAGTTCGAGATCGAGGGCGAGGGCGAGGGCCGCCCCTACGAGGGCCACAACACCGTGAAACTGAAGGTGACCAAGGGCGGCCCCCTGCCCTTCGCCTGGGACATCCTGTCCCCCCAGTTCCAGTACGGCTCCAAGGTGTACGTGAAGCACCCCGCCGACATCCCCGACTACAAGAAGCTGTCCTTCCCCGAGGGCTTCAAGTGGGAGCGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAAGACTCCTCCCTGCAGGACGGCTGCTTCATCTACAAGGTGAAGTTCATCGGCGTGAACTTCCCCTCCGACGGCCCCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCACCGAGCGCCTGTACCCCCGCGACGGCGTGCTGAAGGGCGAAATCCACAAGGCCCTGAAGCTGAAGGACGGCGGCCACTACCTGGTGGAGTTCAAGTCCATCTACATGGCCAAGAAGCCCGTGCAGCTGCCCGGCTACTACTACGTGGACTCCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTGGAGCAGTACGAGCGCACCGAAGGCCGCCACCACCTGTTCCTGGGATCCGGCGCAACAAACTTCTCTCTGCTGAAACAAGCCGGAGATGTCGAAGAGAATCCTGGACCGACCGAG To construct pLentiCRISPRv2-Stuffer-HepmCherry-Puro and pLentiCRISPRv2-Stuffer-HepmTurquoise2-Puro, mCherry and mTurquoise2 were amplified from pKC027 and mTurquoise2-CMV (gifts from Iain Cheeseman), respectively, for 25 cycles with Q5 HotStart Polymerase (New England Biolabs) using the following primers: pLC_EBFP2_F: 5′-GGTTCTAGAGCGCTGCCACCATGGTGAGCAAGGGCGAGGAG-3′ pLC_EBFP2_R: 5′-GCCGGATCCCTTGTACAGCTCGTCCATGCC-3′ Amplicons and pLentiCRISPRv2-Stuffer-HepdsRed-Puro were digested with XbaI and BamHI HF (New England Biolabs) and purified, ligated, transformed, and DNA was isolated and sequence verified as above.

Techniques: Plasmid Preparation, RNA Sequencing Assay, CRISPR, Sequencing, Clone Assay, Amplification, Recombinant, Knock-Out, Software

Journal: Cell genomics

Article Title: Genome-scale CRISPR screening in a single mouse liver

doi: 10.1016/j.xgen.2022.100217

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Plasmid: pLentiCRISPRv2-Stuffer-HepmTurquoise2 , This paper , Addgene Catalog #: 192826.

Techniques: Plasmid Preparation, RNA Sequencing Assay, CRISPR, Sequencing, Clone Assay, Amplification, Recombinant, Knock-Out, Software